The Croyle Lab

The laboratories of Maria A. Croyle RPh., PhD.
The University of Texas at Austin's College of Pharmacy

Dr Maria A. Croyle RPh., PhD is a Professor of Pharmaceutics at The University of Texas at Austin College of Pharmacy where she has lead a busy research lab in pursuit of a single-dose, long-lasting Adenovirus-based Ebola vaccine, as well as studies in drug metabolism, novel vaccine formulations and other viral vectors.
Her students have come from all over the world to be challenged by the plethora of skill sets represented in such an innovative research environment.

Peer Reviewed Scientific Publications

47.  Jonsson-Schmunk. K., Wonganan P., Choi JH., Callahan SM., Croyle, M.A.  Integrin Receptors Play a Key Role in the Regulation of Hepatic Cytochrome P450 3A.  2016.  Drug Metab Dispos. May;44(5):758-70. doi: 10.1124/dmd.115.068874. Epub 2016 Feb 11.
  ** Figures from this manuscript were selected for use as cover art for the May 2016 issue of the journal.

46.  Jonsson-Schmunk. K., Croyle, M.A.  A Long-Lasting, Single-Dose Nasal Vaccine for Ebola: A Practical Armament for an Outbreak with Significant Global Impact.  2015.  Expert Review of Anti-infective Therapy. 13:5 , 527-530.

45.  Jonsson-Schmunk. K., and Croyle, M.A.  A Respiratory Vaccine for Ebola: Close to a Reality?  2015.  Future Virology. 10(6):659-662.

44.  Choi, J.H., Schafer, S.C., Freiberg, A.N.,  Croyle, M.A.  Bolstering Components of the Immune Response Compromised by Prior Exposure to Adenovirus: Guided Formulation Development for a Nasal Ebola Vaccine.   2015.  Mol. Pharm. 12 (8):2697–2711.
    ** Editor’s Choice Award from the American Chemical Society in recognition of the excellent science outlined in the paper and the novelty in which it addresses a current, very serious global issue.

43.  Choi, J.H., Jonsson-Schmunk. K., Qiu, X., Shedlock, D.J., Strong, J., Xu, J.X., Michie, K.L., Audet, J., Fernando,  L., Myers, M.J., Weiner, D., Bajrovic, I., Tran, L.Q., Wong, G., Bello, A., Kobinger, G.P., Schafer, S.C., Croyle, M.A.  A Single Dose Respiratory Recombinant Adenovirus-Based Vaccine Provides Long-Term Protection for Non-Human Primates from Lethal Ebola Infection.  2015.  Mol. Pharm. 12 (8):2712–2731.
    ** Editor’s Choice Award from the American Chemical Society in recognition of the excellent science outlined in the paper and the novelty in which it addresses a current very serious global issue.

42.  Wonganan, P., Jonsson-Schmunk, K., Callahan, S.M., Choi, J.H. and Croyle, M.A.  Evaluation of the HC-04 Cell Line as an In Vitro Model for Mechanistic Assessment of Changes in Hepatic Cytochrome P450 3A During Adenovirus Infection.  2014.  Drug Metab. Dispos. 42(7):1191-1201.

41.  Choi, J.H., Schafer, S.C., Zhang, L., Juelich, T.J., Freiberg, A.N., and Croyle, M.A.  Modeling Pre-Existing Immunity to Adenovirus in Rodents: Immunological Requirements for Successful Development of a Recombinant Adenovirus Serotype 5-Based Ebola Vaccine.  2013.  Mol. Pharm. 10(9):3342-3355.

40.  Leggiero, E., Astone, D., Cerullo, V., Mazzaccara, C., Labruna, G., Sacchetti, L., Salvatore, F., Croyle, M.A. and Pastore, L.  A PEGylated Helper-Dependent Adenoviral Vector Expressing Human Apo-A1 Reduces Atherosclerosis in LDLR Deficient Mice. 2013.  Gene Ther. 20(12):1124-1130.
39.  Choi, J.H. and Croyle, M.A.  Emerging Targets and Novel Approaches to Ebola Virus Prophylaxis and Treatment.  2013.  BioDrugs, 27(6), 565-583.

38.  Choi, J.H., Dekker, J.D., Schafer, S.C., John, J., Whitfil, C.E., Petty, C.S., Haddad, E.E. and Croyle, M.A.  Optimized adenovirus-antibody complexes stimulate strong cellular and humoral immune responses against an encoded antigen in naive mice and those with preexisting immunity.  2012. Clin. Vaccine Immunol. 19(1), 84-95.

37.  Choi, J.H., Schafer, S.C., Zhang, L., Kobinger, G.P., Juelich, T.J., Freiberg, A.N., and Croyle, M.A.  A Single Sublingual Dose of an Adenovirus-based Vaccine Protects Against Lethal Ebola Challenge in Mice and Guinea Pigs.  2012.  Mol. Pharm. 9(1), 156-167.

36.  Wonganan P, Clemens CC, Brasky K, Pastore L, and Croyle M.A. Species Differences in the Pharmacology and Toxicology of PEGylated Helper-Dependent Adenovirus. Molecular pharmaceutics. 2011;8(1):78-92. doi:10.1021/mp100216h.

35.  Wonganan, P., Clemmens, C., Leggiero, E., Brasky, K., Dekker, J., Astone, D., Pastore, L. and Croyle, M.A. Pharmacology and Toxicology of PEGylated Helper-Dependent Adenovirus in Non-Human Primates. Mol Pharm. 2011 Feb 7;8(1):78-92. doi: 10.1021/mp100216h. Epub 2010 Sep 23.

34.  Renteria, S. S., Clemens, C.C., Wonganan, P.W., and Croyle, M. A. Effects of Concentration and Formulation on Adenovirus Stability and Infectious Titer During Actuation from Two Intranasal Delivery Devices.  Vaccine. 2010 Feb 25;28(9):2137-48. doi: 10.1016/j.vaccine.2009.12.025. Epub 2009 Dec 29

33. Wonganan, P., and Croyle, M.A.  PEGylated Adenoviruses: From Mice to Monkeys.  2010. Viruses.  2(2): 468-502 doi:10.3390/v2020468.

32.  Croyle, M.A.  Long-Term Virus-Induced Alterations of CYP3A-Mediated Drug Metabolism: A Look at the Virology, Immunology and Molecular Biology of a Multi-Faceted Problem.  2009.  Expert Opin. Drug Metab. Toxicol. 5(10), 1189-211.

31.  Wonganan, P., Zamboni, W.C., Strychor, S., Dekker, J.D. and Croyle, M.A.  Drug-Virus Interaction: Effect of Administration of Recombinant Adenoviruses on the Pharmacokinetics of Docetaxel in a Rat Model.  2009 Cancer Gene Therapy 16(5):405-14.

30.  Richardson, J.S., Yao, M.K., Tran, K.N., Croyle, M.A., Strong, J.E., Feldmann, H., and Kobinger, G.P. Enhanced protection against Ebola virus mediated by an improved adenovirus-based vaccine. 2009 PLoS ONE. 4(4):e5308.

29.  Croyle, M., Patel, A., Tran, K., Gray, M., Zhang, Y., Strong, J., Feldmann, H., and Kobinger, G.P. Nasal Delivery of an Adenovirus-Based Vaccine Bypasses Pre-Existing Immunity to the Vaccine Carrier and Improves The Immune Response In Mice. 2008 PLoS One. 3(10):e3548.

28.  Callahan, S. M., Wonganan, P., and Croyle, M. A. Molecular and Macromolecular Alterations of Recombinant Adenoviral Vectors Do Not Resolve Changes in Hepatic Drug Metabolism During Infection. Viorlogy Journal2008 Sept. 30 5:111   doi: 10.1186/1743-422X-5-111.

27.  Boquet, M.P., Wonganan, P., Dekker, J.D., and Croyle, M.A.  Influence of Method of Systemic Administration of Adenovirus on Virus-Mediated Toxicity: Focus on Mortality, Virus Distribution, and Drug Metabolism.  J. Pharmacol. Toxicol.  2008. 58(3):222-32.

26.  Callahan, S. M., Wonganan, P., Obenauer-Kutner,  L. J., Sutjipto, S., Dekker, J.D., and Croyle, M. A.  Controlled Inactivation of Recombinant Viruses with Vitamin B2  J. Virol. Meth. 2008 148(1-2):132-145.

25.  Patel, A., Zhang, Y., Croyle, M., Gray, M., Strong, J., Feldmann, H., Wilson, J.M. and Kobinger, G.P. Mucosal Delivery of Adenovirus-Based Vaccine Protects Against Ebola Virus Infection. 2007 J. Infect. Dis. 196(Suppl. 2):S413-S420. 

24.  Le, H.T., Boquet, M.P., Clark, E.A., Callahan, S.M. and Croyle, M.A.  Renal Pathophysiology after Systemic Administration of Recombinant Adenovirus: Changes in Renal Cytochromes P450 Based upon Vector Dose.  Hum. Gene Ther.  2006. 17(11):1095-1111.

23.  Callahan, S.M., Boquet, M.P., Ming, X., Brunner, L.J. and Croyle, M.A.  Impact of Transgene Expression on Drug Metabolism Following Systemic Adenoviral Vector Administration.  J. Gene Med. 2006. 8(5):566-576.

22.  Le, H. T., Yu, Q. C., Wilson, J. M. and Croyle, M. A. Utility of PEGylated Recombinant Adeno-Associated Viruses for Gene Transfer.  J. Control. Rel. 2005 108(1):161-177.

21.  Croyle, M. A., Le, H., Linse, K., Ming, X., Toietta, G., Beaudet, A. and Pastore, L. PEGylated Helper-Dependent Adenoviral Vectors: Highly Efficient Vectors with an Enhanced Safety Profile. Gene Ther. 2005 12(7):579-588.

20.  Callahan, S.M., Ming, X., Lu, S.K., Brunner, L.J., and Croyle, M.A.  Consideration for Use of Recombinant Adenoviral Vectors: Dose Effect on Hepatic Cytochromes P450.  J. Pharmacol. Exp. Ther.  2005. 312(2):492-501.

19.  Croyle, M. A. Callahan, S. M., Auricchio, A., Schumer, G., Wilson, J. M., Linse, K., Brunner, L. J. and Kobinger, G. P. PEGylation of a Vesicular Stomatitis Virus G Pseudotyped Lentiviral Vector Prevents Inactivation in Serum. J. Virol.  2004 78(2):912-921.

18.  Quick, K. S. Gerding, K. and Croyle, M. A. Role of Container/Closure System and Formulation on Agitation-Induced Aggregation Phenomena in Recombinant Adenoviral Products. BioProcessing. 2003 2(5):35-41.

17.  Cheng, X., Ming, X. and Croyle, M. A. PEGylated Adenoviruses Enhance Gene Delivery to the Intestinal Epithelium by the Oral Route. Pharm. Res. 2003 20(9):1444-1451.

16.  Lehmberg, E., Shabram, P., Schluep, T., Wen, S.-F., Sugarman, B., Croyle, M., Koehl, M., Bonfils, E., Malarme, D., Sharpe, G., Nesbit, H., Borellini, F., Kamen, A., Hutchins, B. and Vellekamp, G. Adenovirus Reference Material - Determination of Particle Concentrations obtained by orthogonal, physical - chemical methods.  BioProcessing. 2003 2(3):50-56.

15.  Adadevoh, K. Croyle, M. A., Malarme, D. Bonfils, E. and Bowe, M. A.  A Short Term Field Use and Shipping Stability Study of a Wild Type Ad5 Adenoviral Reference Material.  BioProcessing 2002 1(3):62-69.

14.  Croyle, M. A., Chirmule, N., Zhang, Y. and James M. Wilson. PEGylation of E-1 Deleted Adenovirus Vectors Allows Significant Gene Expression upon Re-administration to Liver. Human Gene Therapy 2002 13(15):1887-1900.

13.  Croyle, M.A., Cheng, X. and Wilson, J.M. Development of Formulations that Enhance the Physical Stability of Viral Vectors for Human Gene Therapy Gene Therapy 2001 8(17):1281-1291.

12.  Croyle, M.A., Cheng, X., Sandhu, A. and Wilson, J.M. Development of Novel Formulations that Enhance Adenoviral-Mediated Gene Expression to the Lung In Vitro and In Vivo. Molecular Therapy 2001 4(1):22-28.

11.  Zhang, Y., Chirmule, N., Gao, G.P., Qian, R., Croyle, M., Joshi, B., Tazelaar, J., and Wilson, J.M. Acute Cytokine Response to Systemic Adenoviral Vectors is Mediated by Dendritic Cells and Macrophages.  Molecular Therapy (2001) 3(5):697-707.

10.  Croyle, M. A., Chirmule, N., Zhang, Y. and James M. Wilson. "Stealth" Adenoviruses Blunt Cell Mediated and Humoral Immune Responses Against the Virus and Allow for Significant Gene Expression upon Re-administration for Gene Therapy to the Lung. J. Virol. (2001) 75(10):4792-4801.

9.  Croyle, M. A., Yu, Q. C. and Wilson, J. M. Development of a Rapid Method for the PEGylation of Adenoviruses with Enhanced Transduction and Improved Stability Under Harsh Storage Conditions. Human Gene Therapy. (2000) 11:1721-1730.

8.  Yu QC, Ma X, Croyle M, Qu G, Huang J, Gao GP, Hughes J, Wilson JM.  Electron microscopic evaluation of vector quality for gene therapy.  Microsc Microanal(2000)  6:858-859

7.  Croyle, M. A., Anderson, D. J., Roessler, B. J., and Amidon, G. L.  Development of a Highly Efficient Purification Process for Recombinant Adenoviral Vectors for Oral Gene Delivery.  Pharm. Dev. Technol. 3(3) (1998) 365-372.

6.  Croyle, M. A., Roessler, B. J., Davidson, B. L., Hilfinger, J. M., and Amidon, G. L.  Factors that Influence Stability of Recombinant Adenoviral Preparations for Human Gene Therapy Pharm. Dev. Technol. 3(3) (1998) 373-383.

5.  Croyle, M.A., Roessler, B. J., Hsu, C-P., Sun, R. and Amidon, G. L.  Beta Cyclodextrins Enhance Adenoviral-Mediated Gene Delivery to the Intestine.  Pharm. Res. 15(9) (1998) 1348-1355.

4.  Croyle, M.A., Stone, M., Amidon, G. L., and Roessler, B. J. In vitro and In vivo Assessment of Adenovirus 41 as a Vector for Gene Delivery to the IntestineGene Therapy, 5 (1998) 645-654.

3.  Croyle, M.A., Walter, E., Janich, S., Roessler, B. J., and Amidon, G. L..  Role of Integrin Expression in Adenovirus-Mediated Gene Delivery to the Intestinal Epithelium.  Human Gene Therapy 9(4) (1998) 561-573.

2.  Walter, E., Croyle, M. A., Davidson, B. L., Roessler, B. J., Hilfinger, J. M., Amidon, G. L.  Adenovirus-mediated Gene Transfer to Intestinal Epithelial Cells as a Potential Approach for Oral Delivery of Peptides and Proteins. J. Control. Rel. 46 (1997) 75-87.

1.  Walter, E., Croyle, M. A., Roessler, B. J., and Amidon, G. L. The Absence of Accessible Vitronectin Receptors in Differentiated Tissue Hinders Adenoviral-Mediated Gene Transfer to the Intestinal Epithelium In VitroPharm. Res. 14(9) (1997) 1216-1222.

Invited Book Chapters

2.  Kobinger, G.P., Croyle, M.A., and Feldmann, H.. Filoviruses.  In: Vaccines for Biodefense and Emerging and Neglected Diseases, Eds.  Barrett, A. D. T. and Stanberry, L. R. New York: Elsevier 2008pp.323-336.

1.  Croyle, M.A.  Gene Therapy.  In: Crommelin, D. Sindelar, E. and Meibohm B., Eds. Pharmaceutical Biotechnology: Fundamentals and Applications. 3rd Ed.  New York: Informa healthcare. 2007 pp:175-210.

Updated:  July 19, 2017 by SCS